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Blood donor white blood cell reduction filters as a source of human peripheral blood–derived endothelial progenitor cells
Author(s) -
Teleron Amylynn A.,
Carlson Brian,
Young Pampee P.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04191.x
Subject(s) - progenitor cell , peripheral blood mononuclear cell , vasculogenesis , endothelial progenitor cell , andrology , neovascularization , immunology , angiogenesis , cord blood , stem cell , ex vivo , medicine , biology , in vivo , in vitro , microbiology and biotechnology , biochemistry
BACKGROUND: Neovascularization in tumors, wounds, and sites of ischemic injury occurs by both angiogenesis (proliferation from existing vessels) and by vasculogenesis (differentiation into endothelial cells from circulating endothelial progenitor cells [EPCs]). EPCs can be obtained from marrow, from cord blood, or by ex vivo expansion of human peripheral blood (PB). The ease of obtaining human PB EPCs has led many recent studies to utilize PB EPCs. The ability to obtain large numbers of PB EPCs would greatly facilitate characterization to further our understanding of EPC biology and their application in cellular gene therapy. STUDY DESIGN AND METHODS: Peripheral blood mononuclear cells (PBMNCs) from whole blood or from the material obtained from white blood cell (WBC) reduction filters were isolated. The cells from both sources were then cultured separately under defined conditions to quantify EPC yield from each source. RESULTS: The yield of EPCs per million PBMNCs plated was approximately 3.5‐fold higher from fresh PB. Because greater numbers of PBMNCs were obtained from each filter, however, the mean yield of EPCs from one filter versus fresh blood was 5.4 million versus 0.4 million, respectively (approx. 14‐fold increased yield). CONCLUSION: The use of WBC reduction filters provides a safe, inexpensive, and readily available source for large numbers of PBMNCs from which culture‐expanded EPCs can be generated for further study.