Premium
Automated preparation of platelet concentrates from pooled buffy coats: in vitro studies and experiences with the OrbiSac system
Author(s) -
Larsson S.,
Sandgren P.,
Sjödin A.,
Vesterinen M.,
Gulliksson H.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04096.x
Subject(s) - buffy coat , platelet , in vitro , medicine , chemistry , immunology , biochemistry
BACKGROUND: The aim was to evaluate platelet concentrates (PCs) prepared by the automated OrbiSac system, from pooled buffy coats (BCs) stored in a platelet (PLT) additive solution. STUDY DESIGN AND METHODS: Experiment 1 was a paired in vitro study of PCs (from six B Cs ), prepared by automated and manual procedures. Experiments 2 and 3 evaluated PCs from OrbiSac (from six B Cs ); Experiment 3 included selection of BCs based on donor data. Experiment 4 was a paired in vitro study of PCs (from six B Cs ) with an integrated white blood cell (WBC) filter and two different storage containers. Experiment 5 evaluated PCs (from six B Cs ) from the OrbiSac with an integrated WBC filter. Experiment 6 was similar to Experiment 5 with computer‐selected pools of 5 B Cs . The in vitro studies evaluated the effects of 7‐day storage of PLTs regarding PLT metabolism and disintegration. RESULTS: Experiments 1 and 4 had similar in vitro results. In Experiment 2, PLT content was 370 × 10 9 ± 70 × 10 9 per PC and recovery from BCs was 76 ± 6 percent. In Experiment 3, the PLT content was 380 × 10 9 ± 50 × 10 9 per PC and variation was reduced compared with randomly pooled BCs. In Experiment 5, increased PLT content was found (420 × 10 9 ± 70 × 10 9 per PC and recovery from BCs of 80 ± 5%). In Experiment 6, five rather than six B Cs gave 340 × 10 9 ± 60 × 10 9 PLTs per PC and recovery was 79 ± 5 percent. CONCLUSION: These in vitro studies suggest that the OrbiSac technique is equivalent to the standard manual method regarding the PLT in vitro characteristics during storage for 7 days. The results of standardizing the PLT count in PCs by selecting the BCs pools on the basis of the blood donor PLT concentration were encouraging.