Premium
Removal of exogenous (spiked) and endogenous prion infectivity from red cells with a new prototype of leukoreduction filter
Author(s) -
SowemimoCoker Samuel,
Kascsak Regina,
Kim Anzi,
Andrade Fabiola,
Pesci Susan,
Kascsak Richard,
Meeker Clifford,
Carp Richard,
Brown Paul
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.00640.x
Subject(s) - infectivity , western blot , virology , biology , whole blood , scrapie , endogeny , bioassay , immunology , microbiology and biotechnology , andrology , medicine , virus , disease , pathology , prion protein , gene , biochemistry , genetics
BACKGROUND: Two recent probable cases of transmission of a variant of human Creutzfeldt‐Jakob disease (vCJD) through blood transfusion suggest that the disease can be transmitted through transfusion of blood components from presymptomatic blood donors. In the absence of a preclinical screening test, removal of the infectious agent by processing is the only means by which risk to recipients of blood from donors with inapparent vCJD infections can be eliminated. STUDY DESIGN AND METHODS: In the endogenous infectivity study, a pool of 500 mL of whole blood was collected into CP2D anticoagulant from 263K‐strain scrapie‐infected hamsters, processed into 300 mL of red cells (RBCs), and then passed through a prion removal filter. Pre‐ and postfiltration samples were tested for PrP sc by Western blot and for infectivity by inoculation of healthy hamsters. In the exogenous (spiking) infectivity study, 30 mL of 10 percent (wt/vol) scrapie‐infected brain homogenates was added to 270 mL of human RBCs and then filtered. Levels of PrP sc and infectivity were determined by Western blot and bioassay. RESULTS: In the endogenous infectivity study, the prefiltered RBCs transmitted disease to 6 of 43 animals, whereas the postfiltered RBCs did not transmit disease to any of 35 animals, and a barely visible prefiltration PrP sc Western blot signal was reduced below the level of detection in the postfiltration sample. In the exogenous (spike) study, infectivity was reduced by 3.7 log LD 50 per mL, from 9.2 to 5.5 log LD 50 per mL. CONCLUSION: The new filter was effective in removing both infectivity and PrP sc from RBCs. The use of this type of filter should reduce the risk of vCJD transmission through blood transfusion.