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Quantification of human platelet antigen‐1a antibodies with the monoclonal antibody immobilization of platelet antigens procedure
Author(s) -
Bertrand Gérald,
Jallu Vincent,
Gouet Maxime,
Kjaer Killie Mette,
Lambin Patrick,
Husebekk Anne,
Kaplan Cécile
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.00195.x
Subject(s) - antigen , serial dilution , antibody , monoclonal antibody , logistic regression , neonatal alloimmune thrombocytopenia , platelet , immunology , medicine , linear regression , fetus , biology , pregnancy , mathematics , pathology , statistics , alternative medicine , genetics
BACKGROUND: Fetal‐neonatal alloimmune thrombocytopenia results from a maternal alloimmunization against fetal platelet (PLT) antigens. In Caucasian persons, human PLT antigen‐1a (HPA‐1a) is the most frequently implicated antigen. Our aim was to develop a method supported by a mathematical approach to quantify HPA‐1a antibodies. STUDY DESIGN AND METHODS: The monoclonal antibody immobilization of PLT antigens (MAIPA) protocol was applied on serial dilutions of a serum with high concentration of HPA‐1a antibodies used as reference. Two mathematical procedures were used: the standard curve was constructed with a linear and a logistic regression. These regressions were used to calculate the amount of antibody in 14 test serum samples from mothers with detectable anti‐HPA‐1a. RESULTS: Similar quantifications were obtained with both mathematical procedures. In 11 serum samples, the results, expressed in arbitrary units (AU), ranged from 62 ± 4 to 1096 ± 19 with the linear regression and from 62 ± 4 to 1117 ± 38 with the logistic regression. In addition, the linear regression allowed us to measure 3 test serum samples with low antibody concentrations from 4 ± 2 to 44 ± 3 AU, whereas the logistic regression was not suitable for the quantification of antibodies in these serum samples. CONCLUSION: A simple quantification method was developed for anti‐HPA‐1a, based on the MAIPA procedure, allowing further studies concerning correlations between anti‐HPA‐1a quantification and clinical relevance.