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STAR: a novel high‐prevalence antigen in the Scianna blood group system
Author(s) -
HueRoye Kim,
Chaudhuri Asok,
Velliquette Randall W.,
Fetics Susan,
Thomas Rebecca,
Balk Mary,
Wagner Franz F.,
Flegel William A.,
Reid Marion E.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2004.04226.x
Subject(s) - proband , antigen , biology , microbiology and biotechnology , exon , antibody , genetics , epitope , mutation , gene
BACKGROUND: More than 20 years ago, a proband was described whose red blood cells (RBCs) typed Sc:1,−2,3. His serum sample contained an immunoglobulin G alloantibody that reacted with all RBCs tested except his own, his brother’s, and those with the Sc:−1,−2 phenotype. Cloning of the SC gene allowed determination of the molecular basis associated with this novel high‐prevalence antigen. STUDY DESIGN AND METHODS: Samples from frozen storage were obtained from the proband, his serologically matched brother, and 15 serologically mismatched family members. DNA was extracted, and amplified products from all 11 SC ( ERMAP ) exons and their flanking regions of the proband were sequenced. RESULTS: A single‐nucleotide mutation was detected (139G>A) in Exon 3 that is predicted to encode a change of Amino Acid 47 from glutamic acid to lysine. The sequence analyses on samples from family members were as expected. CONCLUSIONS: The absence of the high‐prevalence antigen STAR detected by the proband's antibody is likely associated with lysine at Position 47 of the Sc glycoprotein. This amino acid change is located on the extracellular portion of HERMAP, 10 residues upstream from the polymorphism associated with Sc1 and Sc2 (Gly57Arg). STAR expands the Sc blood group system to five antigens and has been assigned the ISBT Number 013005 (SC5).