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Prolonged platelet preservation by transient metabolic suppression
Author(s) -
Badlou B.A.,
IJseldijk M.J.W.,
Smid W.M.,
Akkerman J.W.N.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2004.04022.x
Subject(s) - platelet , von willebrand factor , basal (medicine) , chemistry , fibrinogen , thrombin , energy charge , metabolic activity , medicine , endocrinology , andrology , biochemistry , biology , receptor , diabetes mellitus , adenylate kinase , biological system
BACKGROUND: In this study whether metabolic suppression can be used to preserve platelet (PLT) function during prolonged storage was investigated. STUDY DESIGN AND METHODS: Washed human PLTs were incubated without glucose and with antimycin A to block energy generation. Metabolic suppressed PLTs (MSPs) were stored for 72 hours at different temperatures to find the optimal storage temperature. Controls were incubated with 5 mmol per L glucose and stored at 22 and 4°C. RESULTS: Following metabolic recovery with glucose, MSPs stored at 37, 22, and 4°C showed an increase in basal P‐selectin expression (PSE) reaching greater than 40 percent after about 2, 20, and 48 hours; a decrease in thrombin receptor–activating peptide SFLLRN (TRAP)‐induced PSE inversely related to the increase in basal PSE; and a decrease in TRAP‐induced aggregation reaching less than 30 percent after about 4, 24, and more than 72 hours. When compared with control suspensions, MSPs stored at 4°C better preserved a low basal PSE and in addition showed a better adhesion to surface coated‐von Willebrand factor and fibrinogen in a flow chamber. CONCLUSION: Metabolic suppression before storage at 4°C contributes to better preservation of PLT function.