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Storage of platelets in additive solution for up to 12 days with maintenance of good in‐vitro quality
Author(s) -
Van Der Meer Pieter F.,
Pietersz Ruby N.I.,
Reesink Henk W.
Publication year - 2004
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2004.04010.x
Subject(s) - chemistry , plasma concentration , plasma , in vitro , platelet , platelet poor plasma , chromatography , platelet rich plasma , biochemistry , medicine , physics , quantum mechanics
BACKGROUND: Storage of PLT concentrates (PCs) may be extended beyond 5 days, provided in‐vitro and in‐vivo variables allow longer storage and bacterial screening is performed. The aim of this study was to examine in‐vitro storage characteristics of PCs in various storage solutions: plasma only, or mixtures of plasma with PAS‐II, PAS‐III, PAS‐IIIM, and Composol. STUDY DESIGN AND METHODS: PCs from five pooled buffy‐coats and WBCs reduced by filtration were stored in 1.3‐L butyryl‐tri‐hexyl‐citrate‐plasticised PVC containers. First, a paired comparison was made between PAS‐II and Composol, with 35‐percent final plasma concentration (n = 10). Then, plasma, PAS‐III with 30‐percent plasma, PAS‐IIIM with 20‐ and 30‐percent plasma, and Composol with 20 and 30‐percent final plasma concentration were compared (n = 5 pairs). Finally, 50 PCs in Composol with 35‐percent final plasma concentration were studied. RESULTS: PCs in PAS‐II or Composol had a mean ± SD pH of 6.95 ± 0.09 and 6.96 ± 0.08 at Day 12, respectively. For PCs in PAS‐IIIM and Composol with 30‐percent final plasma concentration, pH on Day 7 was 7.00 ± 0.02 and 6.83 ± 0.05. With 20‐percent final plasma concentration, pH was 6.98 ± 0.02 and 6.81 ± 0.03 for PAS‐IIIM and Composol, respectively. PCs in PAS‐III with 30‐percent plasma had a pH on Day 7 of 6.87 ± 0.03, whereas PCs in 100‐percent plasma had a pH of 7.05 ± 0.03. PCs in Composol with 35‐percent plasma maintained pH greater than 6.8 in 48 of 50 of the units (96%), averaging 7.00 ± 0.10 on Day 8. CONCLUSION: In‐vitro quality of PCs in AS with at least 35‐percent plasma can be maintained for 7 to 12 days after collection.