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Effect of thrombopoietin alone and a combination of cytochalasin B and ethylene glycol bis(β‐aminoethyl ether) N,N′‐tetraacetic acid‐AM on the survival and function of autologous baboon platelets stored at 4°C for as long as 5 days
Author(s) -
Valeri C. Robert,
Ragno Gina,
Marks Peter W.,
Kuter David J.,
Rosenberg Robert D.,
Stossel Thomas P.
Publication year - 2004
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2004.03326.x
Subject(s) - baboon , thrombopoietin , platelet , ethylene glycol , chemistry , ether , andrology , medicine , haematopoiesis , biology , microbiology and biotechnology , organic chemistry , stem cell
BACKGROUND: PLTs stored at 22°C have the potential for bacterial contamination, a problem that could be reduced by 4°C storage. Nevertheless, PLTs stored at 4°C exhibit a significantly reduced life span. This study was performed to determine whether treatment of PLTs with thrombopoietin or cytochalasin B plus ethylene glycol bis(β‐aminoethyl ether) N,N′‐tetraacetic acid (EGTA)‐AM could prevent exponential loss of PLTs stored at 4°C. STUDY DESIGN AND METHODS: Autologous baboon PLTs were stored at 22 or 4°C. The 4°C stored PLTs were treated with 1.5 ng per mL thrombopoietin, with 1 µmol per L cytochalasin B, and 80 µmol per L EGTA‐AM (cyto‐EGTA) or not treated and labeled with 111 In‐oxine to study their in vivo recovery and life span. PLT function was assessed by correction of an aspirin‐induced prolonged bleeding time. Aggregation responses and morphology were also assessed. RESULTS: PLTs stored at 22°C had normal in vivo recovery and linear survival. PLTs stored at 4°C, whether or not they were treated with thrombopoietin, had normal recovery and exponential survival. Aggregation of cyto‐EGTA‐treated PLTs was similar for PLTs stored at 4°C and fresh PLTs, but decreased in PLTs stored at 22°C for 5 days. The addition of cyto‐EGTA to PLTs before 4°C storage inhibited morphologic changes that occurred in PLTs stored at 22°C and cold‐induced PLT clumping, but did not prevent exponential disappearance of the PLTs. CONCLUSION: Addition of thrombopoietin or cyto‐chalasin B and EGTA‐AM to PLTs before 4°C storage did not prevent exponential loss of PLTs.