Premium
Effect of holding buffy coats 4 or 18 hours before preparing pooled filtered PLT concentrates in plasma
Author(s) -
PérezPujol Sílvia,
Lozano Miguel,
Perea Dolores,
Mazzara Roberto,
Ordinas Antonio,
Escolar Ginés
Publication year - 2004
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2004.00641.x
Subject(s) - andrology , buffy coat , chemistry , fibrinogen , trypan blue , whole blood , blood product , leukoreduction , platelet , medicine , immunology , chromatography , surgery , biochemistry , in vitro
BACKGROUND: Filtered PLT concentrates (PCs) were prepared in plasma pooling three (for children) or six buffy coats (BCs; for adults) after holding them a maximum of 4 hours (blood bags collected in the afternoon) or 18 hours (blood bags collected in the morning). STUDY DESIGN AND METHODS: With flow cytometry, PCs prepared after holding BCs 4 or 18 hours were compared. BCs removed from whole‐blood donations in quadruple bag packs (“top‐top”) were held 4 or 18 hours before pooling them with a sterile connecting device. After the BCs were centrifuged, the supernatant was transferred through a BC filter (Autostop, Pall Medical) to a CLX bag. Samples for analysis were collected from the whole‐blood bag, BCs, and PCs immediately after preparation and after 1, 3, 5, and 7 days of storage on a flat‐bed agitator at 22 ± 2°C. The main PLT membrane glycoproteins (GPs, IIb‐IIIa, IV, and Ibα), some of their ligands (fibrinogen, fibronectin, and VWF), activation‐dependent antigens (CD62P and CD63), and procoagulant activity markers (annexin V and bound coagulation FV‐Va) have been studied. RESULTS: In the 12 PCs (six pools of 3 units each group) studied, a minor increase in activation markers during preparation was observed. During the storage, a significant increase in the expression of GPIIb‐IIIa, CD62P, CD63, annexin V, and FVa was measured. After 5 days of storage, only the percentage of PLTs with bound fibrinogen was significantly greater in PCs prepared after holding BCs for 4 hours. CONCLUSION: In PCs prepared after holding BCs 4 or 18 hours before pooling and filtering, only a minor significant difference in the percentage of PLTs with bound fibrinogen was found after 5 days of storage. This difference is probably of little, if any, transfusional significance.