Premium
Some Components of the Kinin System in Cohn's Unheated Human Plasma Proteins
Author(s) -
Morichi S.,
Izaka K.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1976.tb00444.x
Subject(s) - kinin , chemistry , human plasma , kininogen , blood proteins , kallikrein , enzyme , bradykinin , biochemistry , chromatography , receptor
The presence of a kininase and a kininogenase was demonstrated in unheated human plasma proteins. The kininase was found to inactivate synthetic brady kinin and a kinin‐like substance released from a kininogen‐like material in unheated plasma proteins by human urinary kallikrein. It was heat‐labile and inhibited by EDTA, HgCl 2 and 1,10‐phenanthroline. This enzyme was estimated to be kininase I from its inhibition pattern. Inactivation of the kininase revealed the presence of a kininogenase. Five per cent heat‐treated human plasma protein solution has been demonstrated to have no kininase activity but to contain the kinin‐like substance. A mechanism for the formation of the kinin‐like substance in 5 per cent heat‐treated plasma protein solution was discussed in terms of these kinin forming components present in the unheated plasma proteins.