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Chloramphenicol Antibody Causing Interference in Antibody Detection and Identification Tests
Author(s) -
Beattie K. M.,
Ferguson S. J.,
Burnie K. L.,
Barr R. M.,
Urbaniak S. T.,
Atherton P. J.
Publication year - 1976
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1976.tb00443.x
Subject(s) - chloramphenicol , antibody , agglutination (biology) , titer , preservative , lysis , chemistry , reagent , immunology , antibiotics , medicine , biochemistry , food science
Investigation of the serum of three patients with positive antibody detection tests demonstrated the cause in each to be an antibody against chloramphenicol, a bacteriostatic agent used in commercial red blood cell reagents. Washing of these red cells prior to use prevented agglutination. All three examples of anti‐chloramphenicol antibody were IgM and were in low titer when tested at room temperature and 37 C in saline. Two of the antibodies bound complement. The possibility of an antibody to an ingredient of the commercial preservative solution should be considered if problems are encountered in tests with unwashed commercial red blood cell reagents.