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The Purification of Red Cells for Transfusion by Freeze Preservation and Washing
Author(s) -
Crowley J. P.,
Valeri C. R.
Publication year - 1974
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1974.tb04518.x
Subject(s) - platelet , fresh frozen plasma , blood preservation , red cell , red blood cell , whole blood , andrology , blood transfusion , chemistry , medicine , packed red blood cells , surgery , immunology , biochemistry
In the study of 125 units of washed, previously frozen red blood tells approximately 96.6 per cent of the leukocytes and 98.9 per cent of the platelets were removed. Less than 0.5 mg of plasma protein remained per unit. The length of storage as whole blood at 4 C prior to glycerolization of the red blood cells influenced the number of leukocytes in the washed, previously frozen red blood cells. Red blood cells frozen on the day of collection had 7.2 per cent residual white cells, whereas those stored at 4 C for 10 days or longer prior to glycerolization, freezing, and washing had 1.1 per cent residual leukocytes. When red blood cells were stored at 4 C for longer than one week, glycerolized, and washed without freezing, they had about 2 per cent residual leukocytes and platelets. The washing procedure removed at least 95 per cent of the platelets from nonfrozen and previously frozen red blood cells. Because of the removal of large numbers of leukocytes and platelets during the freeze‐preserva‐tion procedure and the excellent recovery of the red blood cells, washed, previously frozen glycerolized red cells are recommended for patients requiring red blood cells depleted of leukocytes, platelets, and plasma protein.