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Pseudomonas Septicemia in Neutropenic Dogs I. Treatment with Granulocyte Transfusions
Author(s) -
Epstein R. B.,
Waxman F. J.,
Bennett B. T.,
Andersen B. R.
Publication year - 1974
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1974.tb04484.x
Subject(s) - leukopenia , pseudomonas , cyclophosphamide , bacteremia , sepsis , medicine , granulocyte , clearance , neutropenia , bacteria , immunology , microbiology and biotechnology , biology , chemotherapy , antibiotics , urology , genetics
A canine model was developed for the study of Pseudomonas septicemia in the leukopenic host. Dogs rendered leukopenic by the intravenous administration of cyclophosphamide were challenged intravenously with 10 7 pseudomonas. An initial period of apparently unimpaired bacterial clearance was followed by a second wave of bacteremia beginning within 24 hours. All leukopenic dogs died during this second phase, in contrast to normal animals which cleared the bacteria and remained well thereafter. Granulocytes suitable for transfusion purposes were obtained by a technique of continuous leukofiltration through nylon columns. The infusion of such granulocyte preparations in quantities of 2 to 15 × 10 9 cells resulted in a demonstrable reduction in circulating numbers of bacteria in septic dogs. The system provides a model for the further study of the natural course of Pseudomonas sepsis in the leukopenic host, as well as a means for evaluating various modes of therapy.