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The Influence of Dialysis in 2‐Mercaptoethanol Reduction of Erythrocyte Antibodies
Author(s) -
Rosner E. R.,
Pirofsky B.,
Sheth K.
Publication year - 1974
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1974.tb04483.x
Subject(s) - antibody , 2 mercaptoethanol , dialysis , reduction (mathematics) , medicine , chemistry , immunology , biochemistry , mathematics , geometry
Sera obtained from 103 normal individuals of blood group O, A, and B were tested in dialyzed and undialyzed 2‐mercaptoethanol reduction procedures. The same results were obtained with both technics with 94 per cent of anti‐A and/or anti‐B antibodies tested. In addition, identical results were obtained by both methods with 31 incomplete erythrocyte antibodies. The two reduction procedures had a similar range of sensitivity. It is suggested that dialysis is not necessary when 2‐mercaptoethanol reduction is employed to differentiate between IgG and IgM antibodies.