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Uptake and Retention of Adenine Moiety by Stored Human Red Blood Cells
Author(s) -
DeVenuto F.,
Wilson S. M.,
Shields C. E.
Publication year - 1973
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1973.tb05474.x
Subject(s) - moiety , chemistry , adenine nucleotide , red blood cell , isotonic , metabolism , red cell , biochemistry , nucleotide , chromatography , biophysics , stereochemistry , biology , medicine , gene , computer security , computer science
Tritium‐labeled adenine was added to freshly collected human blood and at selected storage intervals the distribution of the adenine moiety between plasma and erythrocytes was determined. The retention of the radioactive label by erythrocytes was studied by repeated extractions with isotonic saline. The results show that the adenine moiety, which is utilized for net synthesis of various nucleotides, does not appear to be in a steady‐state equilibrium between plasma and red cells. With increasing time of storage, the equilibrium is shifted toward the erythrocytes which reach a maximum radioactive content at 21 days of storage. Specific association of the adenine moiety with red cell populations of different density, investigated by density distribution of erythrocytes with polyester mixtures of known specific gravity, shows that cell populations of low density (young cells) have a high specific activity.