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Leukocyte Contamination of Red Cells in Leukocyte‐Poor and Frozen‐Deglycerolized Units
Author(s) -
Perkins H. A.,
Senecal I.,
Howell E.
Publication year - 1973
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1973.tb05473.x
Subject(s) - centrifugation , filtration (mathematics) , differential centrifugation , contamination , white blood cell , centrifuge , chromatography , andrology , chemistry , biology , immunology , medicine , microbiology and biotechnology , mathematics , ecology , statistics , physics , nuclear physics
Red blood cell recovery was better and removal of white cells equally good when units were centrifuged briefly in an upright position as compared to more prolonged centrifugation in an inverted position. Nylon fiber filtration left more white cells than did differential centrifugation; a combination of the two technics provided the smallest amount of white cell contamination. Red blood cells frozen and deglycerolized by the original Huggins process had as many leukocytes as those prepared by differential centrifugation. The current Huggins process left more white cells than did the original method. Removal of white cells from blood frozen and deglycerolized by the Huggins process was better in a continuous flow centrifuge than in the Cytoglomerator. Platelet removal was less complete than that of white cells by either technic. Preliminary results with CPD blood do not appear significantly different from those with ACD blood.