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Comparison of Inverted Centrifugation, Saline Washing, and Dextran Sedimentation in the Preparation of Leukocyte‐Poor Red Cells
Author(s) -
Tenczar F. J.
Publication year - 1973
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1973.tb05471.x
Subject(s) - centrifugation , dextran , saline , chromatography , chemistry , andrology , medicine , immunology , anesthesia
Three methods (inverted centrifugation; inverted centrifugation followed by continuous‐flow washing in 5 per cent dextrose‐saline; and dextran sedimentation followed by continuous‐flow washing in 5 per cent dextrose‐saline) were evaluated for the preparation of leukocyte‐poor red blood cells. Residual leukocyte contents were 0.32, 0.40, and 0.16 billion per 100 g of erythrocytes and erythrocyte losses were 24, 42, and 35 per cent, respectively, for the three methods. The mean removal of leukocytes per unit after inverted centrifugation was 80 per cent. Continuous‐flow washing after inverted centrifugation did not remove additional leukocytes. Dextran sedimentation removed a mean 92 per cent of leukocytes. Residual dextran was not detected in the terminal wash solution. These findings suggest dextran sedimentation may be useful for preparing leukocyte‐poor red blood cells for preventing sensitization to leukocytes in high risk recipients such as transplantation candidates.