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A Method for Removing Leukocytes from Stored Blood
Author(s) -
Halterman R. H.,
Yanchulis E.,
Woodruff P.,
Yankee R. A.
Publication year - 1973
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1973.tb05438.x
Subject(s) - buffy coat , platelet , filtration (mathematics) , blood preservation , heparin , packed red blood cells , whole blood , chromatography , chemistry , immunology , medicine , andrology , blood transfusion , surgery , statistics , mathematics
A method for preparing leukocyte‐poor blood from packed red cells that have been stored in ACD for ten days is described. The addition of heparin and CaCl 2 to buffy coat poor‐packed cells followed by nylon fiber filtration removes approximately 97 per cent of the leukocytes when compared with whole blood. Fresh plasma and platelets are useful by‐products of this procedure.