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Bacteriological Study of Platelet Concentrates Stored at 22 C and 4 C
Author(s) -
Mallin W. S.,
Reuss D. T.,
Bracke J. W.,
Roberts S. C.,
Moore G. L.
Publication year - 1973
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1973.tb04476.x
Subject(s) - anaerobic exercise , platelet , contamination , food science , microbiology and biotechnology , chemistry , bacterial growth , platelet transfusion , bacteria , medicine , biology , immunology , physiology , ecology , genetics
Samples of platelet concentrate prepared by double plasmapheresis of volunteers were stored at 4 C and 22 C for four to ten days and cultured from 0072 hours on brain heart infusion media, thioglycolate broth, and tryptic soy broth and incubated at 22 and 37 C for 21 days. A closed system was used for 42 units and a limited entry system involving pooling of platelet concentrates was used for 68 units. All 110 units were negative for bacterial contamination at both 4 C and room temperature, based on cultures using multiple media under maximized growth conditions for anaerobic as well as aerobic bacteria. Platelet concentrates handled in both a closed system and a limited entry system and stored up to at least 72 hours at room temperature remained free of bacterial contamination and appeared safe for transfusion.