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Use of Escherichia coli O 86 : B7 in the Adsorption of Anti‐A and Anti‐B from Blood Typing Sera
Author(s) -
Øyen R.,
Colledge K. I.,
Marsh W. L.,
Wainfan E.
Publication year - 1972
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1972.tb05892.x
Subject(s) - escherichia coli , typing , avidity , adsorption , titer , antiserum , microbiology and biotechnology , bacteria , antibody , chemistry , antigen , blood type (non human) , biology , blood typing , immunology , biochemistry , genetics , organic chemistry , gene
The Gram‐negative bacterium, Escherichia coli O 86 :B7, has somatic A and B antigens that can be utilized to adsorb anti‐A and anti‐B from blood typing sera. Adsorption of anti‐B is highly effective, one adsorption with heat‐killed bacteria for one hour being sufficient to remove anti‐B from most type A or O sera. Adsorption of anti‐A is less efficient, especially with type O sera. Adsorption with these organisms caused slight loss in activity of an anti‐P 1 serum, but no reduction in antibody titer or avidity was detected in adsorptions using a wide range of other human antisera. The bacterial method of processing is a simple and rapid means whereby sera such as anti‐Lu b , anti‐k and anti‐Vel may be rendered free of anti‐B.