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Preliminary Evaluation of an Automated Disposable Continuous Centrifugal Washing System for Frozen‐Stored Blood
Author(s) -
Orlina A. R.,
Brim L. H.,
Gilbert R.,
McDonald B. J.,
Josephson A. M.
Publication year - 1972
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1972.tb00040.x
Subject(s) - chromatography , dilution , liter , osmole , mannitol , chemistry , surgery , medicine , biochemistry , physics , thermodynamics
A new automated continuous‐flow washing system that utilizes disposable wash sets was evaluated for its efficiency in washing bloods glycerolized with equal volumes of Huggins' solution and frozen at −85 C in two‐liter bags. An initial gradient dilution with commercially available electrolyte solutions, modified by the addition of NaCl to give the desired osmolarities, was performed, followed by washing with 500 ml of 20 per cent mannitol in 0.45 per cent saline solution and 2,000 ml of the electrolyte solution. The mean RBC recovery in vitro was 84 per cent, with a range of 79 to 91. The breakdown of the 16 per cent loss was: freeze‐thaw, four per cent; dilutional step, nine per cent; washing, three per cent. The mean RBC survival in vivo with one of the solutions tested was 93 per cent, with a range of 88 to 98. This solution was modified to a final glucose concentration of 300 mg/100 ml and the pH adjusted to 7.4 with TRIS buffer. A significantly lower mean RBC survival of 88 per cent was obtained, with a range of 82 to 95. The maximum supernatant hemoglobin observed after post‐wash storage at 4 C for 24 hours was 804 mg/100 ml, and a low of 137 mg/100 ml. The final osmolarity ranged from 277 to 398 mosm/liter. The total processing time for two units is 70 minutes, of which 40 minutes is spent on the automated washing step. The system appears to be well suited for the large scale processing of frozen blood. However, further work is needed to define the optimum processing solutions particularly for the dilutional step during which time the greatest loss of RBC appears to occur. Its use for blood frozen in liquid nitrogen is under current investigation.