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The Occurrence and Binding of 2, 8‐Dioxyadenine in Plasma
Author(s) -
Stern I. J.,
Cosmas F.,
Garvin P. J.
Publication year - 1972
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1972.tb00030.x
Subject(s) - solubility , chemistry , albumin , uric acid , chromatography , incubation , plasma , precipitation , plasma protein binding , kidney , biochemistry , endocrinology , biology , organic chemistry , physics , quantum mechanics , meteorology
By application of a micro‐column procedure small quantities of 2,8‐dioxy adenine (DA) formed after intravenous administration of adenine (Ad) were detected in dog plasma. Errors due to the overlapping absorbancy of 8‐hydroxy adenine which occurred simultaneously in plasma were compensated for by application of simultaneous equations. An isotopic method was also described which was based on the uniquely low solubility of DA. Investigations in vitro indicated that DA was produced from liver slices in linear fashion with time, while only small amounts were produced after long incubation with kidney slices. The binding of DA to human serum albumin was tighter than that described previously for Ad and for uric acid and permitted apparent plasma concentrations to exceed the solubility in water. The results were consistent with the hypothesis that 2,8‐dioxyadenine was formed in the liver and transported in the plasma to the kidney, where precipitation occurred during protein removal and solute concentration.