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Quantitative Blood Typing Profiles of Human Erythrocytes
Author(s) -
Berkman E. M.,
Nusbacher J.,
Kochwa S.,
Rosenfield R. E.
Publication year - 1971
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1971.tb04423.x
Subject(s) - agglutination (biology) , antibody , antigen , typing , abo blood group system , microbiology and biotechnology , antiserum , chemistry , biology , immunology
Automated, sequential, quantitative blood typing has been achieved by modification of two previously described single channel AutoAnalyzers. In each of these channels, agglutination has been potentiated, either with bromelin and polyvinylpyrrolidone (PVP K‐90) or mannitol and protamine sulfate. Phased pulse‐sampling of both erythrocytes and prediluted antisera through micro‐probes and micro T‐fittings allow sampling rates of 80 tests/hour. Fifty‐five erythrocytic antigenic determinants within all major blood group systems have been assayed and quantitative reproducibility was evident in the identity of data for identical twins and, within sibships, identical genotypes. The degree of agglutinability of unrelated Rhl (Rh o or D) and MN phenotypes assayed with IgM antibodies or with lectins is directly related to the strength of the antigen as measured by the amount of antibody bound by test cells. In contrast, when IgG antibodies are sufficiently potentiated by either sugar or PVP, the relation between strength of cell antigen and degree of agglutination for M and Rhl may be reversed.