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Effects of Albumin on the Radio‐immune Assay for Determining the Anti‐Rh o (D) Content of Rh o (D) Immune Globulin
Author(s) -
Esposito V. M.,
Paul E. B.
Publication year - 1971
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1971.tb04415.x
Subject(s) - albumin , bovine serum albumin , diluent , chemistry , chromatography , immune system , antibody , globulin , serology , microbiology and biotechnology , biochemistry , immunology , biology , nuclear chemistry
Bovine serum albumin is used as a diluent component in the radio‐immune assay for the determination of the Anti‐Rh o (D) content of Rh o (D) Immune Globulin preparations. The radio‐immune assay appears to be more sensitive than other serological assays in the response to differences among albumin preparations; lack of duplication of data and results due to nonspecific binding of immunoglobulin may be pronounced. The effects of bovine and human serum albumin from two commercial sources each were studied. Differences were demonstrated by ultracentrifugal analysis, electrophoresis and serological methods. In addition to monomers and polymers of albumin which are common to both preparations one contained other components. Data presented clearly demonstrate that the choice of albumin preparation is especially critical if the single dilution assay is employed as opposed to the multiple dilution assay.