Premium
An Automated Low Ionic Antiglobulin Test
Author(s) -
Valeri C. R.,
Hirsch N. M.,
Runck A. H.,
Szymanski I. O.,
Johnson L. H.
Publication year - 1971
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1971.tb04386.x
Subject(s) - centrifugation , antibody , tonicity , chromatography , flow cytometry , chemistry , hemoglobin , globulin , immunology , medicine , biochemistry
An automated antihuman globulin test has been performed by continuous flow analysis. Cell washing in acidic low ionic medium achieved sedimentation of aggregated cells and removal of supernatant fluid without centrifugation. These cells were subsequently disaggregated with hypertonic antihuman globulin serum, but human antibody‐coated cells remained aggregated and were removed. Remaining red blood cells were hemolyzed, and their hemoglobin concentration was measured colorimetrically. This automated method detected antibodies of the Kell, Duffy, Kidd, and Lewis blood group systems.