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Isolation of a Substance with Blood ‐group P 1 Activity from Human Erythrocyte Stroma
Author(s) -
Marcus D. M.
Publication year - 1971
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1971.tb04368.x
Subject(s) - stroma , silicic acid , glycosphingolipid , abo blood group system , chromatography , chemistry , chloroform , thin layer chromatography , column chromatography , hemolysis , isolation (microbiology) , fraction (chemistry) , biochemistry , biology , immunology , organic chemistry , microbiology and biotechnology , immunohistochemistry
Lyophilized erythrocyte stroma were extracted with chloroform methanol, and a fraction with blood‐group P 1 activity was obtained by chromatography on columns of silicic acid followed by preparative thin‐layer chromatography. The active fraction contained at least two glycosphingolipids, and it is concluded, tentatively, that the erythrocyte P 1 antigen is a glycosphingolipid.