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The Nature of Rh Blocking Antibodies
Author(s) -
Prager M. D.,
Reed J.
Publication year - 1970
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1970.tb00707.x
Subject(s) - sephadex , chemistry , antiserum , antibody , agglutination (biology) , size exclusion chromatography , blocking antibody , chromatography , immunoglobulin g , electrophoresis , biochemistry , immunology , enzyme , medicine , receptor
Five different anti‐D antisera, each demonstrating both a prozone and specific blocking of D‐anti‐D agglutination, were fractionated by a variety of methods including DEAE‐cellulose chromatography, preparative electrophoresis, and Sephadex gel filtration. In fractions with blocking activity, IgG was either the one immunoglobulin consistently present or the only immunoglobulin detectable. The most potent blocking activity was observed in fractions in which IgG had the most cathodic electrophoretic migration. The possibility that blocking reactions might be associated with either low‐molecular‐weight microglobulins or IgM monomers was considered, but no supporting evidence was found. Specific agglutination inhibition of D‐anti‐D was observed also with urine concentrates from donors undergoing active immunization, and IgG was the most prominent immunoglobulin in these preparations.