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Microtitration of Serum Cold Agglutinins
Author(s) -
Feizi T.,
Monger E.
Publication year - 1970
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1970.tb00700.x
Subject(s) - serial dilution , cold agglutinin , titration , titer , chromatography , dilution , calibration curve , chemistry , antibody , mathematics , medicine , immunology , physics , detection limit , thermodynamics , pathology , inorganic chemistry , alternative medicine
In titration of serum cold agglutinins the microliter system was accurate when titration values did not exceed 1: 256. Beyond these dilutions this technic gave falsely high values. The approximate true level of high‐titer antibodies could be determined by reference to a correction calibration curve and a more accurate determination could be made by initial direct volumetric dilution so that endpoints would fall within the accurate range of the method.