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Typing of Fetal Cells Obtained by Amniocentesis
Author(s) -
Parker J. C.,
Taswell H. F.
Publication year - 1968
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1968.tb02440.x
Subject(s) - amniocentesis , amniotic fluid , typing , fetus , rh blood group system , medicine , abo blood group system , obstetrics , agglutination (biology) , bloody , blood type (non human) , andrology , pregnancy , red blood cell , rh isoimmunization , antibody , immunology , prenatal diagnosis , biology , surgery , microbiology and biotechnology , genetics
In 38 of 287 amniocenteses done at the Mayo Clinic the amniotic fluid contained enough erythrocytes to permit blood typing with commercial sera. Simultaneous use of both an acid‐elution technic and the direct Coombs test helped to establish three of the four possibilities for having erythrocytes in bloody amniotic fluid: 1) maternal and fetal erythrocytes of different blood types; 2) maternal erythrocytes only; 3) fetal erythrocytes only; and 4) maternal and fetal erythrocytes of the same blood type. Twenty of the 38 bloody amniotic fluids permitted fetal erythrocyte‐typing that was later verified postnatally in the living newborns. Traumatic amniocentesis in an Rh o (D)‐negative mother with an Rh o (D)‐positive fetus increases the risk of anamnestic rise in maternal antibody levels. Amniotic epithelial cells from seven different nonbloody amniotic fluids were typed by mixed agglutination technic. Although the ABO groupings were accurate as shown by postnatal typing, Rh o (D) typing was unreliable.