Premium
Continuous Flow Cell Washing System
Author(s) -
Schlutz C. A.,
Bellamy D.
Publication year - 1968
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1968.tb02428.x
Subject(s) - chromatography , continuous flow , chemistry , red cell , cell , computer science , biochemistry , physics , mechanics , computer security
Advancements in blood therapy during the past decade have indicated the need for a presterilized disposable cell washing system. Experimental and prototype developments indicate the feasibility of continuous washing for 1) removal of plasma protein from red cell masses, 2) deglycerolization of frozen red cells, 3) removal of cellular debris, and 4) separation of mixed cell populations. A new system has been designed to accomplish these separations. Initial studies indicate a thousandfold concentration reduction of initial supernate per liter of wash solution per unit of blood. Studies using isotopes and rates of dye removal formed the basis of further studies of programs utilizing deglycerolization and density gradient separation of cells. The basis for the development of the system and experimental results obtained from this system are defined.