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Bromelin Treatment of Red Blood Cells
Author(s) -
Milner L. V.
Publication year - 1968
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1968.tb02386.x
Subject(s) - incubation , titer , serial dilution , antibody , incubation period , suspension (topology) , dilution , antibody titer , medicine , red blood cell , chemistry , andrology , chromatography , immunology , biochemistry , mathematics , pathology , physics , alternative medicine , homotopy , pure mathematics , thermodynamics
The data presented shows that the most effective method for the bromelinization of red blood cells when using 0.5% bromelin, is to incubate equal volumes of an 80% suspension of cells in saline with an equal volume of bromelin at 37 C for 15 minutes. Forty per cent of antibodies will give better reactions at some other suspension of red cells and 25% require a different incubation time. Fifteen minutes is the optimum incubation time for the direct test, but 55% of the antibodies tested in this series had a different incubation period. The indirect test gave titers equal to or higher than the direct test with 86.6% of the antibodies tested. It gave titers one dilution higher in 47.2%, two dilutions higher in 8.33% and 3 tubes higher in 2.78% of the antibodies tested.