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The Effect of Several Variables on Cryoprecipitated Factor VIII (AHG) Concentrates
Author(s) -
Pool Judith Graham
Publication year - 1967
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1967.tb05504.x
Subject(s) - chemistry , diluent , saline , neutralization , platelet , chromatography , yield (engineering) , anticoagulant , biochemistry , immunology , medicine , antibody , nuclear chemistry , metallurgy , materials science
Because of current interest in the new CPD anticoagulant and in acidified platelet‐rich plasma for the production of platelet concentrates, the effect of these changes on cryoprecipitation of Factor VIII was investigated. It was found that use of CPD anticoagulant results in Factor VIII concentrates at least as good as those prepared from corresponding ACD plasma. It was also found that cryoprecipitation of Factor VIII is strikingly affected by pH, with the yield being very low at pH 6.0 and rising steadily to a plateau at about pH 6.8, with no further change up to pH 8.0. Acidified plasma can be restored to normal for production of cryoprecipitates by neutralization of the added acid with NaOH. A survey of five possible diluents for the cryoprecipitates showed that normal saline and retained supernatant plasma both function as well as citrated saline; good stability at room temperature was demonstrated for the AHG in all diluents examined.