Premium
Enzymatic Activities, ADP‐Induced Aggregation and Survival in Vivo as Criteria of Platelet Viability during Storage
Author(s) -
Caen J.,
Cousin C.,
Vainer H.,
Michel H.
Publication year - 1967
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1967.tb04855.x
Subject(s) - platelet , centrifugation , in vivo , chemistry , pipette , in vitro , platelet rich plasma , enzyme , andrology , differential centrifugation , biochemistry , biophysics , chromatography , immunology , biology , medicine , microbiology and biotechnology
To better preserve human platelets in hyper‐citrated plasma in siliconized glassware, the authors recommend centrifugation between 7 and 10 C rather than at 4 C and the dispersion of the button of platelets either by the “propipette” method (drawing the supernatant plasma just above the deposited platelets up and down a pipette) or by a mechanical method using a Whirli mixer. With these methods of dispersion, the rise of acid nitro‐phenylphosphatase in platelets and plasma during one day of storage is less than with the use of a scraping method. The in vitro ADP‐induced aggregation is also well preserved at 4 and at 8 C. Electron microscopic studies do not reveal detectable differences in platelet morphology at these temperatures after one day of storage. The use of radiochromium for platelet tagging does not entirely support the above results but this method is probably not the best for measuring platelet viability during storage. One‐day storage at 37 C results in the non‐aggregability of platelets, conspicuous increase in platelet nitrophenylphosphatase and considerable morphological modifications of platelets, as has been observed by Firkin. 7