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Comparison of Immune and Naturally‐Occurring Antibodies as Test Reagents for Blood Grouping Diabetic Patients
Author(s) -
Guy L. Ruth,
Haberman Sol,
Romick Irene,
Felts Mary H.
Publication year - 1967
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1967.tb04854.x
Subject(s) - antibody , abo blood group system , immune system , agglutination (biology) , immunology , medicine , blood grouping , saline
Blood grouping tests were carried out with 1,124 diabetic patients, using both immune and naturally‐occurring antibodies as reagents. The high‐titered immune antibody gave the most reliable results. The low‐titered immune and naturally‐occurring serums often showed weak agglutination and false‐negative readings. If the erythrocytes were saline washed before testing, the agglutinabil‐ity of the cells was improved. The weak and false‐negative blood grouping reactions with blood samples from diabetic patients appear to be due to a water‐soluble substance which interferes with the reactivity of ABO blood group antibodies but not with antibodies of other blood group systems. This inhibitor substance was unrelated to racial influence, sex of the patient, severity of the disease, the controlling drug or the blood group secretor status of the patient. Periodic blood collections from some of these diabetics have shown that the inhibitor in the serum persists at approximately the same concentration as that originally observed.