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The In Vivo Effects of Deglycerolized Agglomerated Erythrocytes Transfused in Multiple Units to Stable Anemic Patients
Author(s) -
Almond Douglas V.,
Valeri C. Robert,
Booth Wiley M.,
Seiler Michael J.,
Sobucki John P.
Publication year - 1967
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1967.tb04849.x
Subject(s) - hemoglobin , hematocrit , medicine , in vivo , in vitro , immunology , andrology , chemistry , biology , biochemistry , microbiology and biotechnology
Ninety‐two units of deglycerolized cells processed by cellular agglomeration were transfused to 25 stable, anemic patients. For controls, 24 units of nonfrozen cells collected in ACD‐A anticoagulant were transfused to nine similar patients. Laboratory and clinical measurements were made in all recipients before, during, and after the transfusion. Data recorded for each unit of deglycerolized cells included length of storage at 4 C prior to freezing, duration of storage at —80 C and storage post‐thaw at 4 C, together with the in vitro loss during processing. Principal differences consisted of higher supernatant hemoglobin concentrations and reduced amounts of cellular hemoglobin in the deglycerolized units. The smaller amount of cellular hemoglobin was due to the in vitro loss incurred during processing. Hematocrit and total hemoglobin changes in the patients who received either nonfrozen or deglycerolized cells were comparable if the difference in the unit cellular hemoglobin was considered. The mode of removal of the nonviable cells appeared to be via an extravascular mechanism. Higher plasma hemoglobin concentrations and lower serum potassium levels were noted in recipients of deglycerolized cells in contrast to patients receiving non‐frozen cells. No adverse effects on renal function were observed after transfusion with either type of cell.