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Relationship between Lipid Fractions of Erythrocytes and Their In Vivo Survival Following Preservation with Glycerol and the Slow Freeze Technic
Author(s) -
Almond Douglas V.,
Valeri C. Robert
Publication year - 1967
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1967.tb04824.x
Subject(s) - glycerol , cholesterol , centrifugation , chemistry , total cholesterol , in vivo , blood preservation , andrology , zoology , biochemistry , biology , medicine , microbiology and biotechnology
Total lipid, cholesterol, and lipid phosphorus determinations were performed on erythrocytes in the following groups: 1) 22 units of deglycerolized erythrocytes, frozen previously at — 80 C for 6–7½ months with glycerol, processed by cellular agglomeration, and stored subsequently at 4 C from 0 to 11 days; 2) 5 units of deglycerolized erythrocytes processed by centrifugation, frozen previously at —80 C for 6–7½ months with glycerol, and stored subsequently at 4 C for 3 to 10 days; 3) 5 units of ACD blood (NIH formula A) collected from healthy donors and stored at 4 C for 1, and subsequently 7 days; 4) 20 cc aliquots of fresh blood from 5 healthy donors collected in 20 mg disodium EDTA. Chromium 51 ‐labeled 10 cc aliquots of the autologous erythrocytes from full units in groups 1) and 2) were used to determine cell survival at the time the lipid assays were done. The 24‐hour posttransfusion survival was compared with the lipid values obtained. No correlation was found between survival or length of post‐thaw storage up to 11 days and any of the lipid measurements. Cholesterol concentrations in deglycerolized cells varied widely in both directions from normal values, but cholesterol concentrations, on the day of transfusion, could be correlated with the duration of pre‐freeze storage at 4 C. Significantly elevated lipid phosphorus values were observed in the deglycerolized cells after post‐thaw storage, whereas mean values for total lipid and cholesterol were comparable to those of the ACD controls stored at 4 C for corresponding periods of time.