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On the Possible Relation of Sialic Acid to the Rh Factor
Author(s) -
Prager Morton D.,
Lowry Mildred E.
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04781.x
Subject(s) - sialic acid , neuraminidase , thiobarbituric acid , chemistry , rh blood group system , papain , biochemistry , neuraminic acid , enzyme , biology , antibody , immunology , lipid peroxidation
An investigation of the sialic acid content of erythrocyte stroma demonstrating Rh activity has been made. The sediment contains 0.6–0.8% sialic acid initially. Sequential treatment by a papain‐chymotrypsin mixture followed by neuraminidase, or treatment in the reverse order, decreases the level to less than 0.1%. At this lower level there is a shift in the absorption maximum for the color which develops in the thiobarbituric acid assay, indicating that contaminants are playing a more important role in color development than sialic acid itself. With good Rh activity still demonstrable in preparations containing sialic acid levels too low to be measured accurately, it may be concluded that this substance probably plays no role of importance in the structure of the Rh antigenic determinant.