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Relationships between in Vivo Survival and (1) Density Distribution, (2) Osmotic Fragility of Previously Frozen, Autologous, Agglomerated, Deglycerolized Erythrocytes
Author(s) -
Valeri C. Robert,
McCallum Linda E.,
Da David
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04778.x
Subject(s) - erythrocyte fragility , isotonic saline , saline , isotonic , fragility , chemistry , glycerol , andrology , immunology , biochemistry , biology , medicine , endocrinology , hemolysis
In 39 autotransfusions, significant correlations were observed between (1) density distribution and (2) osmotic fragility and 24‐hour posttransfusion survival of chromium‐labeled human erythrocytes preserved with glycerol using a slow‐freeze agglomeration technic. The temperature of storage and the volume of isotonic saline used to disaggregate the agglomerated red cell mass were critical; optimum results were observed following storage of the preserved cells at –80 C and with disaggregation of the agglomerated red cell mass of each unit with 250 cc of isotonic saline. These data indicate that preserved cells with a marked increase in density and a marked decrease in osmotic fragility had a decreased posttransfusion survival. Within 24 hours following the infusion of preserved cells characterized by a marked increase in density and a marked decrease in osmotic fragility, temporary sequestration of a portion of cells was observed, with release of these cells noted on the third to fourth day following the transfusion.