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Relationships between Metabolic State and (1) In Vivo Survival and (2) Density Distribution of Previously Frozen Human Erythrocytes
Author(s) -
Valeri C. Robert,
Bond James C.,
McCallum Linda E.
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04777.x
Subject(s) - glutathione , glutathione reductase , hexokinase , pentose phosphate pathway , biochemistry , nucleotide , glycolysis , chemistry , in vivo , enzyme , biology , glutathione peroxidase , genetics , gene
In 57 autotransfusions, relationships between the metabolic state, characterized by levels of ATP, total nucleotide, and components of the hexose monophosphate shunt and (1) in vivo survival and (2) density distribution of previously slowly frozen, deglycerolized, human erythrocytes were evaluated. Nine of these units were deglycerolized using centrifugation, and 48 units were deglycerolized using agglomeration. Neither the ATP level nor the total nucleotide content was correlated with either the 24‐hour or seven‐day chromium survival. The activity of the sulfhydryl enzymes (hexokinase, glucose‐6‐phosphate dehydrogenase, and glutathione reductase) and glutathione stability were significantly correlated with post transfusion survivals. No correlation was observed between either ATP level or total nucleotide content and the density of the preserved cells. The hexokinase level, glutathione reductase level, and glutathione stability were significantly correlated with the density of these cells. On the basis of these observations, it is suggested that the injury induced by these freezing technics is at the level of red cell membrane.