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Immunochemical Studies of the Rh System. IV. Hemagglutination Assay of Antigenic Expression Regulated by Interaction Between Paired Rh Genes
Author(s) -
Gibbs Mary B.,
Rosenfield Richard E.
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04760.x
Subject(s) - hemagglutination , rh blood group system , gene , hemagglutination assay , microbiology and biotechnology , antigen , genotype , agglutination (biology) , biology , gene expression , chemistry , genetics , antibody , titer
Quantitative hemagglutination assays with anti‐Rh1 (Rh 0 or D), anti‐Rh2 (rh′ or C), anti‐Rh3 (rh″ or E) and anti‐Rh4 (hr′ or c) were performed on the erythrocytes of standard donors of known genotype and of members of one family showing segregation and recombination of four complex Rh genes: R 1,2,–3,–4 , R W1,–2,3,‐4 , R −1,–2,‐3,4 , and R −1,2,–3,–4 . Within the pedigree, duplicate and triplicate examples of each genotype reacted identically. Hemagglutination scores provided concordant data but showed, additionally, that (1) the strength of expression of both Rh1 and Rhw1 (Rh 0 or D u ) directly influenced the amount of agglutination obtainable with guinea pig anti‐rhesus serum (anti‐LW), and (2) in this family R −1,–2,–3,5,19 determines more Rh19 reactivity than does R −1,2,–3,5,19 and much more than R 1,2,–3,5,w19 . The gene R −1,2,–3,–4 was found to depress not only the expression of Rh1 and Rh3 determined by the paired gene, as described previously by others, but Rhw1, Rh2 and Rh4 as well. The gene, R 1,2,–3,–4 , was also found to depress the Rh3 and Rh4 antigenic expression of a paired gene but not to the same degree. These findings suggest that interaction between paired Rh genes may account for most, if not all, of the quantitative genotype differences previously attributed to single “gene dosage” and to pertinent portions of the so‐called “cis‐trans” effect. In contrast to Rh1 assays, those for Rh2, Rh3 and Rh4 were associated with parallel log‐probit regression lines, a finding suggestive of more homogeneity in regard to specific binding affinities of these antiserums and antigenic determinants.