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Absorption of Unwanted Antibodies from Sera Containing MNS or Duffy Group Antibodies without the Need for Selecting “Appropriately Negative” Cells
Author(s) -
Issitt Peter D.,
Jerez Gloria C.
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04716.x
Subject(s) - abo blood group system , reagent , antibody , antigen , enzyme , typing , absorption (acoustics) , group a , chemistry , microbiology and biotechnology , immunology , medicine , biochemistry , biology , materials science , organic chemistry , composite material
Anti‐M, anti‐N, anti‐S, anti‐Fy a and anti‐Fy b typing reagents can be prepared from raw sera by absorption with any ficin‐treated cells of the appropriate ABO group since the action of the enzyme destroys M, N, S, Fy a and Fy b antigens. As well as the advantage that cells need not be selected beyond their ABO group the enhancing action of the use of the enzyme in absorption procedures means that the number of absorptions can usually be reduced. Since ficin does not destroy the s or U antigens the modification cannot be used to prepare anti‐s or anti‐U typing reagents.