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Cryogenic Preservation of Whole Blood for Transfusion In Vitro Study of a Process Using Rapid Freezing, Thawing and Protection by Polyvinylpyrrolidone
Author(s) -
Doebbler G. F.,
Sakaida R. R.,
Cowley C. W.,
Rinfret A. P.
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04706.x
Subject(s) - polyvinylpyrrolidone , liquid nitrogen , hemolysis , chromatography , chemistry , blood preservation , materials science , medicine , andrology , polymer chemistry , organic chemistry , immunology
Polyvinylpyrrolidone (PVP) added to whole blood to a concentration of 7% w/v allows rapid freezing to liquid nitrogen temperatures and subsequent thawing with minimum hemolysis (3%). Average molecular weight PVP greater than about 20,000 is effective. Control of cooling and warming, conditions of heat transfer, temperature, and agitation are essential to high recoveries of red cells. A process has been defined in which blood is collected into ACD anticoagulant and PVP and frozen in corrugated aluminum containers with mechanical agitation in liquid nitrogen. Thawing is carried out with mechanical agitation in water at 37 to 45 C. Reproducible in vitro recoveries of 97% have been obtained with volumes of one‐half and one pints.