Premium
A Simple Hemagglutination System Requiring Small Amounts of Red Cells and Antibodies
Author(s) -
Wegmann Thomas G.,
Smithies Oliver
Publication year - 1966
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1966.tb04696.x
Subject(s) - hemagglutination , agglutination (biology) , serial dilution , hemagglutination assay , hemagglutination tests , chromatography , chemistry , microtiter plate , antibody , microbiology and biotechnology , biology , immunology , medicine , titer , alternative medicine , pathology
A method is described which permits dilute red cell suspensions (1/32%) to be used in plastic microtiter plates for detecting hemagglutinins at unusually high dilutions. The use of suitable additives to the hemagglutination mixture enables excellent settling patterns and a high sensitivity to be obtained. Direct agglutination, inhibition, enzyme promoted agglutination, and Coombs tests can all be performed. Quantitative studies demonstrate the discriminatory powers of the technic. The method may be useful for economizing in the consumption of reagents in blood grouping.