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Enhancement of Immunofluorescent Staining of Erythrocytes by Saponin Hemolysis
Author(s) -
Massey Barbara W.,
Klein Sidney J.,
Reilly Emmett B.
Publication year - 1965
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1965.tb02921.x
Subject(s) - saponin , staining , hemolysis , stain , antigen , hemoglobin , antibody , chemistry , microbiology and biotechnology , fluorescence , biology , biochemistry , medicine , immunology , pathology , physics , alternative medicine , quantum mechanics
The demonstration of human blood group antigens by the fluorescent antibody technic has been improved by hemolyzing the red cells with saponin. Hemoglobin has a quenching effect on fluorescence. Its removal resulted in dramatic enhancement of the stain. Saponin proved more effective for this purpose than several other common hemolytic agents, all of which caused nonspecific staining. Antigens A, B, M, and N were readily demonstrated when the saponin step was incorporated into the usual two‐layer staining procedure. Antigen Rh o (D) required the additional enhancement provided by the triple sandwich technic.