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The Age of Human Erythrocytes Lost During Freezing and Thawing with Glycerol Using the Cohn Fractionator
Author(s) -
Valeri C. Robert,
McCallum Linda
Publication year - 1965
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1965.tb02919.x
Subject(s) - erythrocyte fragility , glycerol , red blood cell , andrology , dehydrogenase , population , glucose 6 phosphate dehydrogenase , chemistry , biochemistry , biology , medicine , immunology , hemolysis , enzyme , environmental health
Three technics to identify the age of erythro‐cytes—Fe 50 , glucose‐6‐phosphate dehydrogenase activity, and osmotic fragility—were used to investigate the red cell population lost during glyceroli‐zation, freezing, thawing and deglycerolization, using the Cohn fractionator with the slow freeze technic. The Fe 50 study showed that “young” red blood cells (less than 20 days old) were preferentially retained. Both the glucose‐6‐phosphate dehydrogenase activity and osmotic fragility tests indicated random loss of “young” and “old” erythrocytes. However, neither of these methods can be used to determine the age distribution of the erythrocytes lost.