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The Antiglobulin Reaction on Albumin Enriched Cell Suspensions
Author(s) -
Clayton Edwin M.,
Brown Richard B.,
Bove Joseph R.
Publication year - 1965
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1965.tb01183.x
Subject(s) - albumin , incubation , chemistry , antibody , agglutination (biology) , saline , coombs test , serum albumin , incubation period , immunology , chromatography , microbiology and biotechnology , biochemistry , medicine , biology
The strength of the antiglobulin reaction with cells incubated in saline was compared with a similar reaction when albumin was added before or after incubation. In one case studied a weak rh“(E) antibody was not detected by the antiglobulin test with saline suspended cells and a transfusion reaction occurred. The incompatibility could be found by the antiglobulin test if the incubation mixture included added albumin. In another case an unidentified antibody had consistently weaker antiglobulin reactions after incubation in albumin compared to saline. An investigation of the antiglobulin activity of 40 antibodies showed antiglobulin agglutination scores that were significantly greater after incubation in the presence of albumin rather than saline. These data suggest that the sensitivity of the antiglobulin test is usually increased when antibodies and erythrocytes are incubated with added albumin.