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The Effect of Adenosine, Inosine, and Adenine on the Concentrations of Organic Phosphate and an Electrophoretic Component (B) of Human Red Cells During Storage of Blood in Acid‐Citrate‐Dextrose and Citrate‐Phosphate‐Dextrose *
Author(s) -
Chanutin Alfred,
Curnish Richard R.
Publication year - 1965
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1965.tb01167.x
Subject(s) - inosine , adenosine , chemistry , adenine nucleotide , preservative , biochemistry , adenosine diphosphate , chromatography , nucleotide , biology , immunology , food science , platelet , platelet aggregation , gene
Bloods, collected in ACD and CPD supplemented with varying amounts of adenosine, inosine, adenine, inosine‐adenosine, inosine‐adenine, and adenosine‐adenine, were stored anaerobically at 4 C for five weeks. At weekly intervals, the P org , and an electrophoretic boundary (Component B) of the washed red cells were assayed. Generally, these values were higher in the CPD than in the respective ACD bloods during storage. The P org , and Component B concentrations were maintained at high levels for the ‐ longest periods with CPD bloods supplemented: with 2.0 mmoles adenosine per 100 ml blood and with 1.0 mmole adenosine plus 0.25 or 0.5 mmole adenine. The most rapid decreases in P org , and Component B were observed in ACD and CPD bloods containing comparatively small amounts of adenine. The relative changes of P org , and Component B occasionally varied markedly when bloods from different individuals were stored in a given supplemented preservative. A high degree of correlation between P org , and Component B was noted.