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The Rh o Variant‐D u
Author(s) -
Pittenger Robert G.,
McQuiston Dorothy,
Sturgeon Phillip,
Gunnink Cornelia,
Agnew Jo Ann
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02889.x
Subject(s) - reagent , rh blood group system , coombs test , antigen , serology , chemistry , blood group antigens , chromatography , medicine , immunology , antibody
A simplified test for the D u factor employing a reagent containing 25 per cent serum albumin, the “stick” technic to introduce cells and forceful cen‐trifugation has been given an extensive trial in a routine blood bank operation. A routine preliminary test with anti‐D gave 5,051 negative reactions among 26,753 bloods tested. The D u tube test was then performed on the former with an anti‐CDE reagent in parallel with the indirect antiglobulin test done with anti‐D. All bloods positive by one or both tests were tested further for D antigens by an anti‐D elution technic; they were also tested for Rh‐Hr factors. Among the 126 bloods positive by elution for the D u factor, 122 and 126 were positive respectively by the antiglobulin and the D u tube tests. The two tests were in agreement on all eight of the type ccD u ee bloods found in the study. The D u tube test, as done with anti‐CDE, gave no false negative reactions. It is, thus, concluded that bloods which are negative with this test will be type ccddee and can be labeled “Rh negative tested for D u . Serological factors which might account for sensitivity of the D u tube test's being the equal of the antiglobulin test are considered, as are the probabilities of the existence of D variants so weak that they could not be detected by either test.