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A Two‐Stage Antiglobulin Test for the Detection of ABO Hemolytic Disease of the Newborn
Author(s) -
Cohen I.,
Nelken D.
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02885.x
Subject(s) - serial dilution , hemolytic disease of the newborn (abo) , abo blood group system , antibody , agglutination (biology) , jaundice , medicine , immunology , cord blood , coombs test , rh blood group system , hemolysis , biology , fetus , pregnancy , pathology , alternative medicine , genetics
A two‐stage antiglobulin test using rabbit anti‐human serum and hen anti‐rabbit serum is described. This test gives positive results with cord blood cells in cases of ABO hemolytic disease of the newborn. Optimal dilutions of rabbit anti‐human serum and hen anti‐rabbit serum are of great importance for this test. The serum dilutions are prepared by coating human Rh positive erythrocytes with anti‐Rh antibodies and adding a diluted rabbit anti‐human globulin serum. The cells which are not agglutinated in this serum are then washed and added to different dilutions of a hen anti‐rabbit globulin serum. The serum dilution giving the strongest agglutination is used in the test. Cord bloods from eight babies with ABO hemolytic disease were examined in the two‐stage antiglobulin test. Forty‐two random cord bloods were also tested. Only the blood specimens from the babies with ABO hemolytic disease (increased plasma bilirubin and clinical jaundice treated by exchange transfusion) showed a strongly positive two‐stage antiglobulin test.