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A Methanol‐Polymer Solution for Protection of Erythrocytes at Sub‐Zero Temperatures
Author(s) -
Kliman Allan,
Osthold Margaret,
Simmons Barbara
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02873.x
Subject(s) - hemolysis , methanol , red blood cell , suspension (topology) , chemistry , red cell , chromatography , biochemistry , medicine , immunology , organic chemistry , mathematics , homotopy , pure mathematics
The hemolysis of red cell suspensions caused by freeze‐thaw injury can be prevented by depressing the freezing point of the suspension. Methanol can depress the freezing point of a red cell suspension but itself causes significant hemolysis if the solution: red cell ratio exceeds 1:1. If 10 per cent PVP is included in the methanol solution, hemolysis due to methanol is reduced significantly and the freezing point of the methanol‐PVP‐red cell mixture can be depressed to —20 C. Hemolysis after storage of erythrocytes for 72 hours at —20 C. in 30 per cent methanol 10 per cent PVP was negligible. Antigenic strength of erythrocytes stored for one month was compared to fresh cells and showed little alteration except for the loss of the Le a character. Methanol‐PVP solutions represent an additional approach to the protection of erythrocytes from injury at sub‐zero temperatures.